To compare the genomic regions containing amha and amhbyclones were isolated from a phenotypic male genomic fosmid library and sequenced. Relative coverage of male and female reads are indicated by blue and red lines, respectively. Identification of Y specific sequences in the Nanopore assembly A sync file containing the nucleotide composition in each pool for each position in the reference was generated as described in the previous section, using the Nanopore assembly NCBI accession number SDAW to align the reads.
Ota et al. Identical bases to the reference are represented as dots and the regions targets by TALENs on amhby are highlighted in yellow in the reference sequence. The continued development of nontraditional models is therefore important to an overall understanding of how sex determining mechanisms evolve and are maintained.
Syst Biol. Genetic map construction Rad-Seq reads were also used to construct a genetic map.
To understand how the mechanism of MSD origin could impact the evolution of sex chromosomes, additional empirical studies identifying the origin of MSDs and sex chromosomes are urgently needed. The genus Gekko is perhaps one of the most interesting gekkotan genera with regard to sex determining mechanisms.
Using RAD-sequencing of a family panel, we identified Linkage Group 24 as the sex chromosome and positioned the sex locus in its sub-telomeric region. Fisheries science. Evolution of the Y Sex Chromosome in AnimalsY chromosomes evolve through the degeneration of autosomes.
S7 Table. The location of amhby on tig is indicated by a solid black arrow.
Temperature-dependent sex determination in a gekko lizard. The variety of sex determining mechanisms described for species in each family are also shown. Widespread hybridization in southern Japan between G.