These observations prompted us to assume that CKAP2 does not affect dynein-dependent transport of spindle pole organizing proteins. Antibodies were diluted in 0. Hardwick, Editor. Introduction to Genomics. Chromosome segregation in mitosis is governed by a complex microtubule-based structure arranged in a symmetrical bipolarity non sex chromosome called and gifted in Huntsville centrosomes located at the two spindle poles.
Subscribe to our newsletter. Egg cells all contain an X chromosome, while sperm cells contain an X or Y chromosome. While the sequence on the two homologous chromosomes may not be identical, the chromosomes have variations of the same genes. Share Tweet LinkedIn Email. The centromere divides the chromosome into two segments called "arms.
A sex chromosome is a type of chromosome that participates in sex determination.
Copyright notice. Abstract Integrity of the microtubule spindle apparatus and intact cell division checkpoints are essential to ensure the fidelity of distributing chromosomes into daughter cells. Science : — Cells were harvested, stained with propidium iodide and analyzed by FACS.
C Thirty minutes post-nocodazole release, microtubules are tethered at distinct poles, often with an increase cells with multipolar spindle poles in CKAP2-depleted cells. CKAP2 is involved maintaining the integrity of microtubule nucleation sites in early mitosis to accurately form the spindle poles.
Integrity of the microtubule spindle apparatus and intact cell division checkpoints are essential to ensure the fidelity of distributing chromosomes into non sex chromosome called and gifted in Huntsville cells.
CKAP2 is involved maintaining the integrity of microtubule nucleation sites in early mitosis to accurately form the spindle poles. In the present study, using the human diploid, karyotypically stable colorectal cancer cell line DLD-1, we elucidated the role of CKAP2 in the formation of the spindle pole, correlated its expression with partners that are known to play a role in the spindle formation, and finally investigated the cellular mechanism by which chromosomal instability arises in cells with altered expression of CKAP2.
A—C Mitotic cells were treated with colcemid in order to obtain metaphase spreads. Using an asynchronous population of cells, we monitored the incidence of anaphase lagging chromosomes, micronuclei, and nuclear blebs.
Ultimately, chromosomal instability seems to enable tumor cells to adapt chromosome content to improve their fitness . Maximum intensity projections were taken for each sample and analyzed by ImageJ 1.